Journal: Molecular Therapy. Nucleic Acids

Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters

doi: 10.1016/j.omtn.2025.102612

Figure Lengend Snippet: Screening of optimal ASO candidates targeting SARS-CoV-2 (A) Schematic of the SARS-CoV-2 minigenome luciferase reporter system. The reporter vector contains the SARS-CoV-2 5′ UTR, the firefly luciferase coding region, the SARS-CoV-2 N gene, and the 3′ UTR region of the SARS-CoV-2 genome. (B) ASOs targeting SARS-CoV-2 reduce luminescence in the minigenome reporter system. Relative light units (RLU) were normalized to ASO-C, at each of the indicated concentrations. Relative fluorescence units (RFU) were used as internal transfection normalizer. n = 4; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. (C) RT-qPCR determination of changes in SARS-CoV-2 RNA in infected HEK293t/ACE2 cells treated with PS-ASOs directed to SARS-CoV-2 or control ASO (ASO-C) ( n = 3; ∗ p < 0.05). (A) was created using BioRender.com .

Article Snippet: We wish to thank Dr. Mélanie Lavie from the Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, France, for kindly providing the human ACE-2-expressing HEK293T (HEK293T/ACE2) cell line, Pr.

Techniques: Luciferase, Plasmid Preparation, Fluorescence, Transfection, Quantitative RT-PCR, Infection, Control

Journal: Molecular Therapy. Nucleic Acids

Article Title: Antisense oligonucleotides targeting the SARS-CoV-2 nucleocapsid gene decrease viral titers in hamsters

doi: 10.1016/j.omtn.2025.102612

Figure Lengend Snippet: Combinations (1:1) of RNase-H-dependent and steric-block oligonucleotides act synergistically to decrease SARS-CoV-2 viral titers (A) Oligonucleotides and chemical modifications for antiviral challenges. Deoxyribonucleic acid (DNA), ribonucleic acid (RNA), phosphorothioate (PS), 2′-O-methoxyethyl (2MOE), locked nucleic acids (LNAs), morpholino oligonucleotides (MPOs), and small interfering RNA (siRNA). (B) Chemical modifications or the nucleic acid nature affect targeting effects in the luciferase reporter minigenome in HEK293T-ACE2 cells, n = 4. (C) Transfection of cells with combinations of PS or LNA ASOs with MPOs increases the antiviral effect in VeroE6/TMPRSS2 cells. Individual oligonucleotides or combinations were transfected at a 100 nM total concentration and infected after 3 h with ZsGreen-expressing SARS-CoV-2 virus at an MOI of 0.1. The effect of ASO combinations on the green fluorescence of infected cells was determined at 24 h. (D) Effect of ASO combinations in viral titrations (TCID 50 /mL) in SARS-CoV-2 Wuhan (Hu-1)-infected cells. n = 3. ∗ p < 0.05, ∗∗ p < 0.01. ∗∗∗ p < 0.001.

Article Snippet: We wish to thank Dr. Mélanie Lavie from the Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, France, for kindly providing the human ACE-2-expressing HEK293T (HEK293T/ACE2) cell line, Pr.

Techniques: Blocking Assay, Small Interfering RNA, Luciferase, Transfection, Concentration Assay, Infection, Expressing, Virus, Fluorescence